Data was baseline subtracted and then fit using a sigmoidal dose-response (variable slope) equation.
Half maximal inhibition (IC50) values were calculated by GraphPad® Prism 7 using a sigmoidal nonlinear fit model (4PL regression curve).
Therefore, the hydration kinetic was evaluated using a sigmoidal model (R2 > 0.99).
ELISA data was analyzed with GraphPad Prism 7 using a sigmoidal nonlinear fit (4PL regression curve) model to determine the half maximal Effective Concentration (EC50) serum or antibody titer.
The relationship between baclofen-induced encephalopathy as a function of the baclofen concentrations was described using a sigmoidal Emax model.
Dose response data was fitted using a sigmoidal function with variable slope (Prism 5, GraphPad Software, San Diego, CA).
Concentration response relationships were examined using a sigmoidal concentration response curve.
Concentration-response curves were fitted using a sigmoidal dose response at variable slope (GraphPad Prism, La Jolla, CA).
Dose response relationships were examined using a sigmoidal dose response curve.
Where appropriate, doses were log-transformed and a non-linear regression analysis was applied using a sigmoidal dose response curve.
