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Dose response relationships were examined using a sigmoidal dose response curve.
Values were expressed as a percentage of untreated cells and 50% inhibitory concentration (IC50) values were derived by fitting dose response curves using a sigmoidal dose response equation.
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The EC50 value and curve fitting were performed with GraphPad Prism using a Sigmoidal dose-response (variable slope).
- Dose-response lethality curves and histograms for Mn content measurements were generated using GraphPad Prism (GraphPad Software Inc .. We used a sigmoidal dose-response model with a top constraint at 100% to draw the curves and determine the LD50 values.
Where appropriate, doses were log-transformed and a non-linear regression analysis was applied using a sigmoidal dose response curve.
Therefore, the hydration kinetic was evaluated using a sigmoidal model (R2 > 0.99).
Concentration-response curves were fitted using a sigmoidal dose response at variable slope (GraphPad Prism, La Jolla, CA).
Nonlinear regression was performed on the resulting values using a sigmoidal dose response model with variable slope equation in GraphPad Prism version 4.0b for Mac (Mac 05 version) (GraphPad Software, San Diego, CA).
The effects of maternal diet on logEC50 values and steepness of the curve (Hill slope) for integral activity were analysed by GraphPad Prism (Version 6; GraphPad Inc) using a sigmoidal dose response (variable slope) curve to test the null hypothesis that logEC50 and Hill slope for the same for each data set.
Dose response data was fitted using a sigmoidal function with variable slope (Prism 5, GraphPad Software, San Diego, CA).
Dose response studies were also subjected to regression analysis using a sigmoidal curve fitting model: where EC50 is the median effective concentration.
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