Sentence examples for using a siRNA library from inspiring English sources

Another group using a siRNA library composed of 22,329 pools of siRNA targeting 19,709 genes in HeLa P4/R5 cells detected 311 host factors, with an 18-gene overlap with Brass et al. (Zhou et al., 2008).

We have validated our assay by using a siRNA library preferentially targeting genes involved in cellular transport.

Using a siRNA library directed at the full complement of human DUBs, we identified POH1 (also known as Rpn11 or PSMD14), a component of the proteasome lid, as a critical DUB controlling the apparent ErbB2 levels.

In order to better and more extensively characterize the implication of kinases in the AhR cascade, we have analyzed the consequences of kinase expression knock-down on TCDD-mediated up-regulation of CYP1A1 activity, using a siRNA library covering the human kinome.

We took a synthetic lethal approach using a siRNA library targeting 418 apoptosis-related genes and identified genes and pathways whose inhibition synergized with topotecan.

ROR1 is identified to be important for the survival of ALL cells with t(1 19)(q23;p13) translocation when using an siRNA library for the tyrosine kinome to screen critical tyrosine kinases for ALL pathogenesis (Bicocca et al., 2012).

Validation testing of the 470 primary hits was performed using an siRNA library of deconvoluted pools of four single siRNAs per target.

We used an siRNA library to target 98 DUBs in two cell lines dependent on Mcl1 for survival: IMR5 and NLF.

To further exclude the possibility of "off-target" effects associated with RNAi, we recapitulated this result using a siRNA distinct from the Qiagen library siRNA (Fig. 1B) and two pharmacologic PARP1 inhibitors, 3-aminobenzamide (3-AB) and NU1025 (Fig. 1C).

RNAi screen was performed in 384-well plates using a pooled siRNA library (Dharmacon SMART pool library; Thermo Scientific), where each gene was targeted by a pool of four single siRNAs.

Nevertheless, by using a small siRNA library we have validated the process used to identify dysregulated genes.