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Muscles were then digested in sterile filtered type 1 collagenase (0.2%) in DMEM using a shaking water bath for 2 hours at 37 degrees.
Fluids were maintained at 37°C using a shaking water bath.
Hybridization was performed manually using a shaking water bath/Twincubator® preheated to 45°C.
The temperature of SBF was maintained by using a shaking water bath, and SBF was completely refreshed every day.
Hybridization of the single-stranded, biotin-labeled amplicons to membrane-bound probes on the strip followed by addition of conjugate, and substrate to detect visible band patterns on the strip was performed manually using a shaking water bath, Memmert-SV1422 (Memmert GmbH & CO.KG, Schwabach, Germany) at 45°C.
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The residue was dried over night and then extracted with 250 ml water (H2O) by using a shaking water-bath at 70°C for 2 hours.
Gently agitate the SDS solution using a shaking machine.
In this work, the solubility of cefoxitin acid in organic solvents (methanol, acetonitrile, ethanol, isopropanol, n-propanol and ethyl acetate), water and water-methanol mixtures was measured spectrophotometrically using a shake-flask method within the temperature range 278.15 303.15 K.
This paper reports on a series of experiments using a shake table on a scale model PVC tank containing water.
Plugs were washed thrice at 54°C for 10 minutes with 5 10 ml of water in a shaking water bath and the same washing steps were repeated this time using TE buffer.
Samples were then incubated for 20 minutes at 37°C in a shaking water bath.
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