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Also, since two non-synonymous variants (rs3743930, rs224222) as well as three synonymous variants (rs224225, rs224224, rs224223) were observed in MEFV exon 2 during the sequencing screening phase, genotyping of these variants was done using a sequencing approach.
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Since both the time and cost of sequencing a transcriptome or genome are markedly reduced, researchers are willing to use a sequencing approach to leap over the bottlenecks when conducting a classical genome/EST library sequencing project.
In comparison to cloning-based approaches, using a sequence-only approach further reduces the amount of bench work required.
Prior analysis using a sequence alignment approach coincides with this interpretation (Brown et al. 2002).
The gene environment of the Takifugu, Xenopus, chicken and human PAC1 homologue regions were compared using a sequence similarity approach.
The gene environments of the protostome (C. elegans and Drosophila) and deuterostome (human and Takifugu) receptor genes were compared using a sequence similarity approach.
By using a sequence-based approach, Diavatopoulos et al. analyzed 7 B. holmesii isolates and observed only 1 nonsynonymous polymorphism among 3,666 bases (16 ).
We aimed to sequence the complete cpDNA genome of L. perenne and therefore chose to extract pure cpDNA from a perennial ryegrass population and to sequence it using a shotgun sequencing approach.
The genome P. sabinae T27 was sequenced by using a hybrid sequencing approach that incorporates 454 pyrosequencing with Illumina Genome Analyzer.
We sequenced small RNAs using a multiplatform sequencing approach (ABI SOLiD, Illumina GA II and HiSeq) (fig. 1 A and B).
Orthologous otopetrin sequences were generated using a targeted sequencing approach (from dog, cow, armadillo and western clawed frog) (see methods in [ 13, 14]) or identified through tBlastn searches of available whole-genome sequences.
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