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The filtrate was then partitioned (3×) with equal amount of n-hexane using a separatory funnel, to remove the remaining oil components from the resin.
Learn how to "work up" your reaction using a separatory funnel to perform a liquid-liquid extraction.
Finally, oil and solvent were separated using a separatory funnel.
Filtered culture broths were extracted with an equal volume of ethyl acetate or dichloromethane using a separatory funnel.
Afterwards, 20 ml of water was added, and the TEA was removed using a separatory funnel.
After filtration, the conditioned media was extracted with an equal volume of DCM using a separatory funnel.
The resulting conditioned media was extracted with an equal volume of DCM using a separatory funnel (2 3 times).
This was repeatedly extracted three times with diethyl ether using a separatory funnel in a ratio of 1 : 1 of solvent and ECM.
The hexane extract was subjected to repeated silica gel (0.063 0.200 mm, Merck) column chromatography, using a hexane-ethyl acetate gradient as the eluting solvent.
Elution was carried out using an n-hexane: octane ethyl mixture (60 40% v/v).
The upper hexane layer was separated using a separating funnel and hexane rota-evaporated.
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