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Learn how to "work up" your reaction using a separatory funnel to perform a liquid-liquid extraction.
Finally, oil and solvent were separated using a separatory funnel.
Filtered culture broths were extracted with an equal volume of ethyl acetate or dichloromethane using a separatory funnel.
Afterwards, 20 ml of water was added, and the TEA was removed using a separatory funnel.
After filtration, the conditioned media was extracted with an equal volume of DCM using a separatory funnel.
The resulting conditioned media was extracted with an equal volume of DCM using a separatory funnel (2 3 times).
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The biodiesel was extracted from the filtrate using n-hexane in a separatory funnel, and then glycerol (bottom phase) was separated from the organic phase.
The acidified water phase was thus defatted in a separatory funnel using n-hexane (3 × 25 mL).
After the addition of a surrogate solution (0.2 mL) to the oil sample (0.2 mL), the mixture was moved to a separatory funnel using 10 mL hexane.
For extraction, equal volumes of two phases, organic and aqueous phases, were equilibrated in a separatory funnel by using magnetic stirrer at 200 rpm for 10 min as shown in Fig. 1.
This biphasic solution was stirred for 1 h and transferred to a separatory funnel.
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