First we consider adaptive supertagging, a widely used approximate search technique that prunes most lexical categories from the parser's search space using a separate sequence model.
Dissection of the promoter using a separate sequence analysis tool (MAPPER, http://genome.ufl.edu/mapper/; Marinescu et al., 2005) identified several transcription factor binding motifs (Fig. 2A).
In other respects, this dataset is ideal as a benchmark, as the 454 sequences stem from the same individual on which the reference is based while the reference was constructed using a separate sequence set.
Using a separate computational pipeline that mapped sequence reads directly to the DM reference genome [ 35], we identified 2,117,754 raw SNP calls, of which, 147,525 SNPs remained after filtering with the Bowtie/SAMTools pipeline.
The false identification rate (FIR) of peptide identification was determined by using a separate decoy search using the reversed sequences since the OMSSA E-value is determined by database size.
Always use a separate conditioner.
Some authors have suggested using two separate sequencing platforms on the same samples [ 10], while others have suggested that technical replicates for exon capture may help to further improve accuracy of heterozygote variant calls [ 62].
Approximately 35 50 ng of purified DNA was used in separate sequencing reactions for forward and reverse primers with 90 nM primer, Big Dye Terminator v3.1 (Applied Biosystems, Foster City, CA) and 5× buffer (400 mM Tris pH 9.0 and 10 mM MgCl2, Applied Biosystems) in a final volume of 11 μl.
Two different repetitive TMS (rTMS) protocols were applied to the motor cortex in 10 subjects and the effect on rCBF was measured using a CASL sequence with separate RF coils for labeling the inflowing blood.
We think that this can be done by using the work presented in (Kushik et al. 2014) that handles the derivation of a separating sequence using only the defined inputs of the machine.
