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Ex vivo autoradiography and histology were conducted using a separate cohort of S1R-KO/WT mice, and radiation dosimetry was calculated from WT mouse data (extrapolated for human dosing).
Using a separate cohort of subjects we show that 8 weeks of active control training (ACT) generates the same behavioral impact also through an effect on signals in the vmPFC.
Using a separate cohort of primary tumor and normal tissue, we validated NSCLC promoter hypomethylation and increased expression by quantitative RT-PCR for all 10 genes.
The prospective validation of the score using a separate cohort indicated that score was indeed robust in predicting a nosocomial drug resistant gram negative infection.
The outcome of the analysis was then validated retrospectively, under an institutional review board-approved protocol from Dana Farber/Harvard Cancer Center (protocol no. 12-519), using a separate cohort of patients who were treated with NovoTTF-100A and bevacizumab at Beth Israel Deaconess Medical Center.
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We then used a separate cohort of 11 normal lung tissues from patients without a cancer diagnosis to confirm epigenetic silencing via promoter methylation in normal lung mucosa from patients without a lung neoplasm.
After we determined the time course of %MPE of morphine on the hot plate, we used a separate cohort of rats to determine baseline latencies to respond to the hot plate following minocycline administration.
We performed a similar analysis using a separate, independent cohort of 13 NSCLC samples with 14 lung samples from patients without neoplastic disease and confirmed promoter hypomethylation in target genes.
To validate our microarray findings, we performed real-time RT-PCR on the three identified candidate biomarker genes using a separate animal cohort with more defined time points to increase validity.
Using a separate prospectively-followed Mayo Clinic Rochester cohort, this study confirms previous findings that ante-mortem PiB binding is associated with accumulation of underlying amyloid-β neuropathology (Lockhart et al., 2007; Ikonomovic et al., 2008; Driscoll et al., 2012).
External validation of the final prediction equations for ESRD and death was performed using information available from a separate cohort of 213 patients with stages 3-5 CKD recruited at a renal unit in East Kent between June 2003 and June 2004.
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