Sentence examples for using a sensitive qPCR from inspiring English sources

Importantly, we tested decontamination using a sensitive qPCR approach targeting small DNA fragments (as small as 73 bp), which allows analysis of highly degraded samples.

All DNA samples were tested for PCR inhibition using a sensitive qPCR approach targeting a segment of exogenously added jellyfish DNA at a concentration suitable for detection of low levels of inhibitors [13].

26 Using a sensitive qPCR method, we detected HIV gag DNA in Ag85A-specific CD4 T cells from only 1/11 subjects postvaccination (three low-dose and eight high-dose).

Use a sensitive moisturizer.

Using highly sensitive qPCR analysis (see Materials and Methods, Fig. S2B and C), significantly increased accumulation of mtDNA deletions was observed in Polg +/D257A mice (Fig. 2E).

In our current studies, using more sensitive qPCR measurements of Gli transcription in different cells (unmodified NIH3T3 cells), we see an enhancement of Hh transduction (in a Neuropilin-dependent manner) with all five of the Sema3 proteins tested.

Investigation of a broader number of BAT miRNA targets using highly sensitive qPCR-array methods will provide a more comprehensive overview of the potential miRNAs regulating BAT development and function.

Lack of introns in the prokaryotic genome makes it challenging to study bacterial gene expression using a highly sensitive technique like RT-qPCR [ 7, 8].

Relative viral fitness was assessed using a highly sensitive, quantitative real time PCR (QPCR) assay to measure production of total HIV-1 DNA.

Using a more sensitive technique with type-specific qPCR primers, Symbiodinium B224 was found in all corals at 3 weeks and in 40% of the B224-treated colonies at 5 weeks (Fig. 5A).

qPCR was carried out using a KAPA SYBR® FAST qPCR Kit (Kapa Biosystems) in the ABI Prism 7300 Sequence Detection System (Applied Biosystems).