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Using a sensitive assay developed to specifically detect recombinant lentiviral DNA mobilization, we demonstrated that the trans-lentiviral vector prevents the generation of recombinants that contain a functional gag-pol structure, while the lentiviral vector generates env-minus recombinant lentivirus that mobilizes recombinant genomes to other cells when pseudotyped with an exogenous envelope.
All samples were also screened for the Δexon14 transcript using a sensitive assay based on RT-PCR with fluorescent fragment analysis.
Based on our data using a sensitive assay serological evidence of viral infection was found in almost 80% of assessed cases [ 11, 12].
Here, using a sensitive assay method, we found a significant increase of sialidase activity in the serum of patients with prostate cancer compared with that in healthy subjects having low activity, if any.
Using a sensitive assay to follow transcripts in living mouse embryos, we found that Cdx2 mRNA becomes preferentially localized apically at the late eight-cell stage and inherited asymmetrically at the eight- to 16-cell transition.
By using a sensitive assay, WB, we showed that a high frequency of IgG antibodies directed to Stx2 was present in the sera of HUS patients and in 62.9% of healthy children.
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Using a sensitive cTnI assay, we studied cTnI concentrations in predialysis patients with CKD who did not have an acute coronary event.
Indeed, using a sensitive antigen assay, LAIR-2 could not be detected in plasma of healthy individuals [9].
Sensitivity of thyroglobulin (Tg) measurement in the follow-up of differentiated thyroid carcinoma (DTC) can be optimized by using a sensitive Tg assay and rhTSH stimulation.
Two patients with recurrent disease could be identified solely on the basis of Tg-on levels ≥1.0 ng/ml by using a sensitive Tg assay.
Using a sensitive PCR assay to detect the HSV-2 UL30 gene (Fig. S2), long term HSV-2 DNA shedding into the vaginal fluids was demonstrated (Fig. 1).
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Justyna Jupowicz-Kozak
CEO of Professional Science Editing for Scientists @ prosciediting.com