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Sections of frontal and parietal cortex, basal ganglia, thalamus, midbrain, cerebellum and brain stem were examined microscopically and scored independently as mild, moderate, or severe and were each given numerical scores of 1 (mild), 2 (moderate), or 3 (severe) by using a semiquantitative system as described [43].
The intensity of CHO-131 mAb staining for C2-O-sLex in immunoreactive tissues was scored using a semiquantitative system from 0+ to 3+.
The nuclear and the cytoplasmic staining patterns were separately quantified, for both phosphorylated RKIP and STAT3, using a semiquantitative system for evaluation and grading of the immunostaining pattern, successfully applied by us and others [ 50, 51].
The density of the inflammatory infiltrate was examined in those sections, and was scored using a semiquantitative system that recorded a value of 0 for lack of inflammation, 1+ for mild inflammation and 2+ for marked inflammation.
Cyclin D1 expression was scored using a semiquantitative system as described by Vos et al. 18 This system was based on the staining intensity scored as 0 (none), 1 (weak), 2 (moderate), and 3 (strong), and the percentage of positive tumor cell nuclei scored as 0 (0%), 1 (1–25%), 2 (25 50%), 3 (50 75%), and 4 (>75%).
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The hepatic fibrosis score was significantly reduced in 63% of IFN-γ treated patients compared with 24.1% in the control group by using a semiquantitative scoring system evaluating both liver architecture and fibrotic deposits.
Back redness (erythema), presence of scales (scaling), and hardness of the skin were scored using a semiquantitative scoring system from 0 to 4 based on their external physical appearance: 0 = no skin abnormalities, 1 = slight, 2 = moderate, 3 = marked, and 4 = severe.
The intestinal injury was evaluated using a semiquantitative scoring system ranging from 0 to 4 modified by Arumugam et al. [33].
Histological evaluation was performed using a semiquantitative scoring system.
Lung changes were analyzed histologically using a semiquantitative scoring system, as previously described elsewhere [ 18].
Nuclear immunostaining results for KDM1A were evaluated using a semiquantitative scoring system.
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