Sentence examples for using a secreted alkaline from inspiring English sources

GR AF1 activity was determined using a secreted alkaline phosphatase (SEAP) assay as described [7].

Transcriptional activation by NF-κB was monitored using a secreted alkaline phosphatase (SEAP) reporter plasmid, pNF-κB-SEAP, which contains four κB consensus sequences upstream of SEAP [50].

Samples were tested 'blind' at the University of Queensland for circulating antibodies to the HPV16/18 late capsid protein L1 using a HPV-neutralising assay based on pseudoviruses carrying a secreted alkaline phosphatase reporter gene as described by Pastrana et al. [ 17].

Wnt throughput was defined using a Wnt pathway reporter construct containing seven LEF/TCF consensus binding sites preceding a secreted alkaline phosphatase (7X-SEAP) reporter.

Using a reporter gene assay we show that HEK-produced IFNα2b is biologically active as it induces the production of a secreted alkaline phosphatase (SEAP) reporter enzyme under the control of the human ISG56 promoter (Fig. 8).

The experiments were conducted using a murine secreted alkaline phosphatase (mSeAP), whose production can be quantified in blood samples as well as on tissue sections.

Based on the theoretical analysis described in the previous paragraph, we used secreted alkaline phosphatase (SEAP) as a reporter protein in order to probe the targeting capacity of the predicted domains of shrew-1's signal peptide.

Gaussia luciferase and secreted alkaline phosphatase activities were measured using a Secrete Pair TM Dual Luminescence Assay Kit following the manufacturer's instructions (GeneCopoeia).

To monitor gene expression, we used as reporter gene a murine secreted alkaline phosphatase (mSeAP).

We found that IL-12 p40 has several features that make it a particularly attractive reporter gene when compared to another secreted protein which is being used widely, that is secreted alkaline phosphatase (SEAP): i) the IL-12 is produced by macrophages and dendritic cells only in response to antigenic stimulation.

Our strategy consisted in the pre-injection of polymers before intravenous administration of mice with AAV2 encoding a murine secreted alkaline phosphatase (mSeAP).