Sentence examples for using a secondary antibody from inspiring English sources

An electrochemical ELISA-like amplification strategy was set up using a secondary antibody conjugated to horseradish peroxidase (HRP).

Monoclonal antibody BM1234 was added to acetone/methanol-fixed P. falciparum cultures of the 3D7 strain, and its binding was detected by confocal fluorescence microscopy using a secondary antibody (green).

We have designed and optimized a classical ELISA in which Epi-hNE4 was coated directly on microtitre plates and the antibodies were detected using a secondary antibody labelled with peroxidase.

Bound antibodies are visualized using a secondary antibody conjugated to colloidal gold particles.

Antibody binding was visualised using a secondary antibody (polyclonal, Cy5TM-conjugated anti-mouse IgG from goat; Zymed).

After overnight coating of a 96-well plate with a primary antibody, TGF-β1 was detected in cell lysates using a secondary antibody.

EnVision uses a secondary antibody against both rabbit and mouse that is directly labelled with HRP (horseradish peroxidase) reacting with DAB, whereas LSAB uses a secondary antibody against rabbit and mouse, labelled with biotin, then streptavidin-HRP is added and the staining is done with DAB.

After washing, Envision + polymer (ready to use; Dako) was used as a secondary antibody.

Primary antibodies were detected using a secondary HRP-conjugated antibody.

DakoEnVision™ + HRP-conjugated anti-mouse antibody was used as a secondary antibody, and no counterstain was employed.

was used as a secondary antibody.