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And that process, in turn, can be made visible using a second enzyme, called luciferase.
The hydrogen peroxide produced can be monitored using a second enzyme, horseradish peroxidase (HRP), and a chromogenic peroxidase substrate.
The amount of H2O2 released is then monitored using a second enzyme, horseradish peroxidase, together with a peroxidase substrate [ 21].
PFGE remains the standard for E. coli genotyping because of its discriminatory power; these results were also confirmed by PFGE by using a second enzyme.
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26 The presence of antibodies to hepatitis C was tested using a second generation enzyme immunoassay (Abbott Laboratories, Chicago, IL) and confirmed by the MATRIX assay (Abbott Laboratories).
A second solution would consist of using a second type IIs enzyme (for example BpiI) to perform the cloning from entry clone to expression vector.
The results used here were obtained using a second generation HCV enzyme immunoassay for most subjects, with a few of the newer recruits having data from a third generation test.
Anti-HCV was using a third-generation enzyme immunoassay (Abbott Diagnostics).
Anti-HCV tests were conducted using a third-generation enzyme immunoassay kit (AxSYM® HCV Version 3.0; Abbott Laboratories, Berkshire, UK).
Serum antibody to hepatitis C virus was assayed using a third-generation enzyme immunoassay kits (HCV EIA III; Abbott Laboratories).
All samples were tested for HBsAg and anti-HCV markers by using a third-generation enzyme immunoassay (Axsym; HCV EIA 3.0; Abbott Laboratories, Abbott Park, Illinois, and HBsAg, Axsym) as previously published [ 14].
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