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GC content analysis of Sirtuin genes were performed in the various organisms using a script implemented by us (Table 1).
The GC levels of compositionally nearly homogeneous DNA segments were calculated using a script implemented by us.
All calculations were performed using a script implemented in the R software, version 2.7.1 http://www.r-project.org.org
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A script implemented by us was used to align the sequences and to extract the insertions/deletions in Venter's chromosomes, considering three size classes (10 100, 100 1000, >1000 bp), as well as the single nucleotide polymorphisms (SNPs).
For the remaining genes a script implemented by us was used in order to identify the coding sequences beginning with a start codon and ending with a stop codon.
For the remaining genes a script implemented by us was used in order to identify the coding sequences beginning with a start codon and ending with a stop codon in order to calculate reliable GC, GC1, GC2 and GC3 values (the GC levels of first, second and third codon position).
We conducted single marker and haplotype TDT association analysis using a custom script implemented in R statistical software and based on Transmit [14], and the equivalent FBAT approach [15] for comparison of the results.
The feature selection was completed using a custom script implemented in SAS.
EGN is a script implemented in Perl.
In addition to discipline parallelization and coarse-grain parallelization of the disciplines, embarrassingly parallel capability to run multiple parameter cases is implemented using a script system.
The residuals from the fit of the model to the data were visualized using the R script implemented in Treemix.
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