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Enzymes with the required activity can then often be identified using a screen based on a coupled enzyme assay.
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Based on our findings with the DSRS and CPSS, we can compare how the instruments would perform in a cost-based analysis using a screening based strategy.
To verify these results using an independent approach, we undertook a screen based on mRNA expression profiles, protein localization, and small hairpin RNA (shRNA) depletion.
Recently, we performed a comprehensive search for hypermethylation events in 16 UM using a screening assay based on methylation-sensitive restriction digest of genomic DNA followed by PCR amplification and array based detection of 323 different CGIs.
The frequency of germline mutants in G1 was sufficient to be used for gene targeting relying on a screen based solely on molecular methods.
The potential risk to cetacean species from direct interaction with fisheries was assessed using a screening procedure based on a Productivity Susceptibility Analysis (PSA).
The studies were further assessed for their relevance using a screening tool based on study design, intervention target and type of intervention.
To surpass such inhibition, several rounds of laboratory evolution in combination with semi-rational approaches were carried out using a screening assay based on the biochemical composition of human blood.
Using a genetic screen based on the inability to propagate the yeast [ PSI+] prion, we have identified 13 new Sse1 mutants that are predicted to alter chaperone function through a variety of different mechanisms.
Given the high sequence divergence of OTU domains, we set out to identify unstudied OTU enzymes using a bioinformatical screen based on generalized profile analysis (Bucher et al., 1996).
CadA has recently been characterized using a functional screening based on heterologous expression of a cosmid library produced from STM 2683 genomic DNA (Maynaud et al., unpublished observation).
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