Sentence examples similar to using a scratch pipette from inspiring English sources

Using a standard pipette tip, a scratch was then introduced through the centre of each well.

The confluent monolayer cultures were treated with or without 0.1 µg/ml tetracycline for 4 hours and were then wounded with a straight scratch using a yellow pipette tip.

Using a p200 pipette tip, scratches were made in triplicate in each well of the confluent monolayer.

Cell lines 1542-NPTXX, 1542-CPC3X, PC3 and DU145) were grown on glass coverslips to confluency and scratched using a plastic pipette tip.

After 24 h, the cells were scratched using a sterile pipette tip, washed twice, and incubated in serum-free medium.

Briefly, 2 × 10 cells where seeded on poly- L-Lysine (Sigma-Aldrich -coated Sigma-Aldrich -coatedlture medium; upon confluency a Sigma-Aldrich -coatedg a P10 pipette tip.

For the wound healing assay, SCAPs were cultured to 90% confluence in 100 mm culture dishes and then wounded by using a pipette tip to scratch the monolayers.

Briefly, cells were wounded by a single scratch using a sterile p200 pipette tip attached to suction, rinsed with phosphate-buffered saline and allowed to migrate in serum-free medium in a humidified incubator at 37°C/5% carbon dioxide.

Thereafter, the monolayer was artificially wounded by using a pipette tip to scratch across the plate; the cells were washed with PBS to remove the detached cells and then cultured in serum-free medium in the presence of mitomyocin C (10 μg/ml) to prevent cell proliferation.

After cells were more than 80% confluent in 6-well plates, the scratch was made using a sterile pipette and then treated with vehicle (DMSO) or compounds.

The cell monolayer was then subjected to a mechanical scratch-wound induced using a sterile pipette tip.