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The bacterial densities were determined using a scattered light turbidimeter.
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Prior to entering the CO2 laser beam, each particle crosses a second, low energy laser beam and produces a scattered light signal used to determine the particle size in real time.
The time to reach a scattered light intensity of 10000 arbitrary units (a.u).
Wave aberrations were measured using back scattered light at 10Hz.
This use of imaging using multiply scattered light improves the visibility of the microvasculature and erythrocytes through two mechanisms.
Fourth, since OCT can measure well-defined volumes using predominantly single scattered light, quantitative assessment of hemodynamic parameters such as blood vessel diameter and blood flow using Doppler algorithms are possible [ 8– 12].
Amplifications were ×40,000 for B, C and D and ×100,000 for E and F. To determine cell viability, flow cytometry (FCM) using scattered light was chosen.
Bennett argued that while the measurement processes discussed by Gabor and Brillouin, using scattered light, generate heat, they were incorrect to generalise this to the claim that all measurement process must necessarily do the same.
When using scattered light the SLOT setup has the same isotropic optical resolution of equivalent OPT setups.
Using scattered light detection with SLOT offers the same isotropic optical resolution of equivalent OPT setups [ 19].
Although this illumination method can be used for scattered light observations as well, we use this method mainly with monochromatic illumination and fluorescence filters recording samples stained with fluorescent markers.
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