The evolution of new phase formation is monitored by time-resolved light scattering using a fibre optic scattering cell as a function of the depth of penetration into the two-phase regions.
Using a log forward scatter versus log side scatter dot plot, a two dimensional analysis gate was drawn around the cancer cell population, and a fluorescence histogram was obtained for 10,000 events for each sample.
The size of the PFC/ICG nanoemulsions was analyzed via dynamic light scattering using an electrophoretic light scattering photometer (ELS-Z, Otsuka Electronics, Osaka, Japan).
The T-lymphocytes were identified and gated using a combination of light scatter parameters (forward scatter and side scatter) and fluorochrome conjugated anti-CD3 antibody fluorescence, following fluorescent antibody labelling of PBL.
Ergül et al. [ 4] measured scattering cross-sections and used a combination of forward scattering, backward scattering, and side scattering in their analysis to differentiate healthy and diseased cells from each other.
Living cells were gated by using a combination of forward scatter and side scatter.
Cellular fragments and debris were excluded from the analysis by using a side-scatter versus forward-scatter histogram to establish a collection gate.
The scatter sinogram was estimated from the emission data and the attenuation maps using a single scatter simulation algorithm [ 28].
The cells were analyzed on forward-angle light scatter and 90° light scatter using a lymphocyte gate.
Exposures were performed by using an anti-scatter grid.
The average hydrodynamic diameter and zeta potential of BTs were determined using dynamic light scattering and phase analysis light scattering, respectively, using a Zetasizer Nano-ZS, Model ZEN3600 (Malvern Instruments Ltd., Malvern, UK).
