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MS/MS analyses were performed using a scan width of m/ z 0.2 and a scan time of 0.125 s.
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Seed length and width were measured using a scanned image analyzing software called SmartGrain (Tanabata et al. 2012).
Samples were analyzed using selected reaction monitoring (SRM) mode with a scan width of 1 m/z and a scan time of 0.05 s.
Product-ion scanning analysis was repeated n-times for molecular ions of m/z = (100+n), n = 0,1,2.251 at a scan width of 0.5 each.
All spectra were recorded using a 200-G scan width.
using a P-value cutoff of 0.001 and a scanning width of 600.
The standard OCT image (B-scan) used for this study contains 900 A-scans, which amounts to a scanning width of 3 mm.
Collision induced dissociation (CID) was induced on the twenty most abundant ions per full MS scan using an isolation width of 1.5 Da.
Samples were scanned 5 times at 50 nm/min, using a band width of 1 nm.
Drift independence is obtained for small critical features using a radial scan strategy, and a back scan routine ensures minimal line width deviation removing redeposited material.
It then scans the genome using a window of width w (default 20) and, for each window, it subtracts the number of antisense reads from the number of sense reads (called net tag count).
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