At the start and end of the treatment period, ocular refractions were measured using retinoscopy and vitreous chamber depth (VCD) and axial length (AL) using A-scan ultrasound.
A total of 23 family members (9 affected and 14 unaffected) participated in this study and had a full ocular assessment to document the phenotype, including visual acuity testing, slit-lamp photography, corneal diameter measurement, and axial length measurement using A-scan ultrasonography.
Spectra were collected from 215 to 260 nm in a 0.2-cm path length quartz cuvette using a scan rate of 20 nm min−1 and a response time of 8 s.
Seed length and width were measured using a scanned image analyzing software called SmartGrain (Tanabata et al. 2012).
Composition, morphologies, length, size, and diameter of the nanorods were studied using a scanning electron microscope and energy dispersed x-ray spectroscopy analyses.
Sample dimensions are measured using a scanning electron microscope, and are used as input in finite element simulations of the bars' elastic deformation for various crack lengths.
To minimise the effects of observer bias, and to address the problems of intratumoral heterogeneity in marker expression, we used a scanning autostage to image the entire length of the core biopsies, and defined regions of tumour tissue using an anticytokeratin cocktail.
Keratometry was performed using Bausch and Lomb keratometer, while the axial length was measured using an A scan biometer (Appasamy associates, Chennai).
Stained slides were scanned using a Mirax SCAN automated slide scanning system (Zeiss).
To assess similarity across the genomes, sequence scans were performed using a fragment length of 300 bases and an increment of bases between fragments.
