The second stage of the algorithm is based on fitting a model for each independently used A-scan (depth-scan) to further improve the accuracy.
This prediction was tested on the nanotube, 170 nm in diameter (Figure 4a), using a scan area (5 × 5 nm), a scan velocity of 12.5 nm/s, and loads of 3, 9, and 15 nN.
Three different scan areas using a scan speed of 20 nm/s were tested (Figure 2c, d).
After hybridization, the microarray slides were washed and scanned using a Scan Array Express Lite (Perkin Elmer Life and Analytical Sciences).
MS/MS analyses were performed using a scan width of m/ z 0.2 and a scan time of 0.125 s.
Samples were analyzed using a UPLC system (UPLC Acquity, Waters Ltd., Elstree, U.K). coupled online to a Q-ToF Premier mass spectrometer (Waters MS Technologies, Ltd., Manchester, U.K). in both positive and negative ion electrospray modes, using a scan range of 50−1000 m/ z and a scan time of 0.08 s.
Microarrays slides were scanned using a Scan array Express system (PerkinElmer), all slides were scanned with set laser power at 90%% and photomultiplier gain at 55%% for Cy5 and Cy3.
The MS was operated in a data-dependent mode, in which one full scan with m/z 400 1600 in the Orbitrap using a scan rate of 30 ms/scan.
Stained slides were scanned using a Mirax SCAN automated slide scanning system (Zeiss).
Photostimulation was carried out using a dual scan head raster scanning confocal microscope and control software developed by Prairie Systems, and incorporated into a BX51 upright microscope (Olympus America).
The sample topography was studied using a scanning probe microscope (SPM) operated as a scanning force microscope in the 'tapping mode' in air (Digital Instruments Nanoscope III multimode).
