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A durotherm (Aw Messer- Germany) was calibrated using a saturated solution of barium chloride and left to stand for 3 h until water activity reading was at 0.900 in an incubator.
Samples were concentrated with ZipTipC4 (Millipore) as described by the manufacturer using a saturated solution of sinapinic acid (Fluka) prepared in an 80% (v/v) of acetonitril/0.3% trifluoroacetic acid for elution.
The peptide mixture was analyzed using a saturated solution of CHCA in 50%acetonitrile/0.1%% TFA.
The mass spectrum was acquired in linear mode, using a saturated solution of sinapinic acid as ionization matrix.
Peptide mixture was analysed using a saturated solution of α-cyano-4-hydroxycinnamic acid (HCCA) in 50%acetonitrile/0.1% % trifluoroacetic acid.
The localization of lignin was determined histochemically on stems of axillary secondary shoots of the main mutant/WT, stem as well as on 4 6 weeks old plantlets, using a saturated solution of phloroglucinol in 20% HCl.
Similar(53)
The reaction mixture was adjusted to pH 9 to 10 using a saturated Na3PO4 solution and was incubated for 16 h at 4°C.
Complete removal of the native Ta2O5 can be attained using a saturated KOH solution that is assisted by an anodic voltage.
The aluminum substrate was then completely removed using a saturated CuSO4 solution with a small amount of HCl (38 wt%), and the AAO template was etched in 6 wt% H3PO4 for 20 h at 23°C as shown in Figure 1a.
Triplet states were quenched in all three systems using a saturated Trolox solution.
The pellet was incubated with proteinase K at 56 °C and subsequently salted out at 4 °C using a saturated NaCl solution.
More suggestions(15)
using a hypotonic solution
using a saturated model
using a sterilizing solution
using a straightforward solution
using a rubberised solution
using a hybrid solution
using a mixed solution
using a standard solution
using a simplified solution
using a saturated barium
using a hosted solution
using a saturated pole-placement
using a saturated transposon
using a bleach solution
using a dye solution
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