The new multi-modal anion-exchangers can also be used at normal anion-exchange conditions and with either a salt step or a pH-step to acidic pH can accomplish the elution of proteins.
The recovery of BSA was also tested with a salt step (from 0.25 to 2.0 M NaCl using 20 mM Piperazine as buffer, pH 6.0) or with a pH-step to pH 4.0.
His6-Arg was purified over an SP-Sepharose column, using a step salt gradient to elute, followed by a Ni affinity column, using ArgEB buffer [20 mM HEPES (pH 7.25), 5 mM β-ME, 500 mM KCl, 0.01% NP-40, 5% glycerol, 100 mM imidazole, and protease inhibitors] to elute.
Use a salt or baking soda rinse.
Use a salt water mouth rinse.
Use a salt-stain remover.
The aim of our study was to prepare a carrier-free dry powder formulation of meloxicam potassium (MP), a novel salt form of meloxicam, using a one-step co-spray drying technology.
The second set of SiNWs, with generally smaller diameters, is fabricated using a two-step MACE process with silver salts [19, 20, 23, 26, 27].
The new multi-modal cation-exchangers can also be used at normal cation-exchange conditions and with either a salt-step or a pH-step (to pH-values where the proteins are negatively charged) to accomplish elution of proteins.
