Sentence examples for using a rubber policeman from inspiring English sources

For the western blot assay cell lysates of hMVEC were prepared by scraping the wells on ice using a rubber policeman and by adding Roche lysis buffer with phosphatase and protease inhibitors (Roche, Mannheim, Germany).

Cells were washed twice with ice-cold phosphate-buffered saline (PBS) and thereafter collected using a rubber policeman.

Whole cellular extracts from control and stimulated cells were prepared from cells detached using a rubber policeman, and harvested from culture medium into Eppendorf tubes.

Cells were washed three times in cold PBS containing 100 µg/ml cycloheximide and were scraped off the plate using a rubber policeman and 1 ml of the same solution.

Cells were scraped using a rubber policeman before addition of another 50 μl lysis buffer.

Scrape the lysed cells from the dish using a rubber policeman.

GAPDH RNA levels were analyzed in parallel to ensure that equal amounts of cDNA were loaded for each reaction Cells were harvested in ice-cold phosphate-buffered saline, pH 7.4, using a rubber policemen, then lysed by addition of an equal volume of 2× sodium dodecyl sulfate (SDS) lysis buffer (100 mM Tris HCl, pH 6.8, 200 mM β-mercapto-ethanol, 4% SDS, 0.2% bromophenol blue, and 20% glycerol).

Using a rubber spatula, fold together until blended.

Using a rubber spatula, fold everything together just until blended.

Using a rubber spatula, fold in bay leaves and almonds.

Fold in the coconut using a rubber spatula.