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Bouin's fluid-fixed tubules were dehydrated in graded ethanol, embedded in paraffin wax using a routine method, and sectioned at 7 µm using a rotary microtome (Microm HM340E).
To assess the quality of sodium bisulfite conversion and the subsequent PCR/pyrosequencing assays, using the LINE-1 marker, we evaluated conversion of 40 FFPE DNAs extracted using a routine method.
Salmonella bacteria were isolated using a routine method [ 36]: briefly faecal samples were enriched aerobically in peptone buffer at 37°C for 24 hours followed by incubation in Rappaport-Vassiliadis broth at 42°C in aerobic conditions again for 24 hours, before inoculation onto Rappaport-Vassiliadis agar and incubation for 24 hours at 37°C.
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Fasting serum total cholesterol, serum HDL cholesterol, and serum triglycerides were measured using a routine enzymatic method (Roche Diagnostics, Mannheim, Germany).
Serum GGT was measured by JPS Laboratories (formerly London, UK) using a routine kinetic colorimetric method.
Immunohistochemical staining for HLA class I antigen expression was performed using a routine avidin/biotin peroxidase method.
Immunohistochemical analysis of CC3, cleaved PARP and MHC-II expression was performed using a routine streptavidin biotin peroxidase method.
Bacteria were cultured on blood agar at 37°C for 24 hours using serial dilutions and counted using a routine plate count method.
Genomic DNA was isolated from peripheral blood of the patients, their parents, and several normal individuals using a routine phenol-chloroform method.
How can medical and social causes and contributory factors be investigated using a routine health surveillance method that utilises the perspectives of final caregivers?
After signing the informed consent, peripheral blood samples from the patients and their parents were collected in EDTA-containing tube followed by DNA extraction using a routine phenol-chloroform method.
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