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The extracted wall residue was then milled in a porcelain jar (1 l) with ceramic balls using a rotatory ball mill running at 96 rpm under nitrogen for 120 h.
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The paraffinized blocks were then sectioned using a rotatory ultra microtome, transferred to glass slides and allowed to dry overnight.
After extraction, these three extracts were mixed and concentrated using a rotatory evaporator and then dried under a stream of high purity nitrogen.
The obtained methanol extract was filtered and evaporated by using a rotatory evaporator and freeze dryer.
The resulting extracts were then concentrated using a rotatory evaporator and freeze-dried.
Serial sections of 2 7 microns thick were obtained using a rotatory microtome.
The embryos were sectioned (transverse sections, 5 µm) using a rotatory microtome RM2255 (Leica, Nussloch, Germany).
Embedded samples were sectioned using a rotatory microtome (HM 325, Microm).
Using a rotatory microtone, sections of 5 μm thick were cut and mounted on clean glass slides.
The resulting filtrate was concentrated by evaporation under vacuum at 40°C, using a rotatory evaporator (Buchi Labortechnik AG, Switzerland).
The extract was concentrated into a small volume using a rotatory evaporator and then freeze-dried to produce a crude extract (CE, 100 g).
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