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Using a rotating bioreactor system with simulated microgravity conditions, the entire mouse inner ear organ can be maintained in culture for up to seven days with preservation of sensory organ morphology and robust marker protein expression in sensory hair cells.
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a Using a rotating line source.
Measurements were performed using a rotating anode generator.
No previous studies have used a rotating wall vessel bioreactor for intervertebral disc explants culture.
Engineers at the US National Aeronautics and Space Administration NASAA) devised a rotating bioreactor, which is a useful device for culturing cells on Earth, as well as in space.
In a rotating bioreactor, these organoapatite-coated foams are successfully colonized by preosteoblastic cells.
Cells were seeded dynamically onto biodegradable scaffolds, which then were maintained in a rotating bioreactor for 6 to 8 weeks.
Differentiated cells grown on a biodegradable polymer scaffold and a rotating bioreactor also exhibit morphologic traits and biomarkers characteristic of liver cells (Wang et al., 2012).
EB-derived cells grown in a rotating bioreactor exhibited higher levels of liver-specific functions than those in static culture (Zhang et al., 2013a).
They simulated the mass transfer in systems in which cultured cells are attached to small microcarriers in a rotating bioreactor in simulated and real microgravity.
To create tumor spheroids, rat osteogenic sarcoma UMR-106 cells were cultured in Dulbecco's modified Eagles' medium and then transferred to a rotating bioreactor where they form spheroids in 7-10 days.
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