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Gel sections (1.5 mm) were excised with a scalpel, extensively washed in deionized water and digested with trypsin (0.25 µg) using a robotic workstation (MassPrep, Micromass).
Then, 2 µl of each test compound or vehicle was transferred from the mother plates to the assay plates using a robotic workstation (BiomekR FX Beckman).
This study describes a novel automated protocol for fast and reproducible DNA library preparation for massive sequencing using a robotic workstation capable of handing carboxylic-acid coated superparamagnetic beads for precipitation of DNA.
All DNA samples were dispensed to 96-well plates using a robotic workstation (Beckman Biomek 2000 with integrated MJ research DNA Engine PCR cycler).
Direct sequencing of PCR-generated templates was done using reactions based on the chemistry of BigDye Terminator v3.0 Cycle Sequencing Ready Reaction Kits (Applied Biosystems, Foster City, California, USA) with reactions set up in 96-well microtiter plates using a robotic workstation.
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Seeding of the reporter cells, addition of lysates and SEAP activity measurement were performed using a robotic pipetting workstation (Microlab Star, Hamilton).
Dilutions and other pipetting procedures were performed using a robotic QIAgility™ workstation (Qiagen).
Samples were prepared using a robotic liquid handling workstation (epMotion 5075, Eppendorf, Germany) and incubated with antibody-coupled beads for 60 min followed by incubation with a detection antibody for 30 min.
We use data collected using a robotic mine detection system.
Printing was performed using a Flexsys robotic workstation equipped with a 384-pin head.
After 60 minutes of incubation, the filter plates containing the bead-immobilized antibody-antigen complexes were washed using a BioMek robotic workstation with a vacuum manifold.
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