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Following 24 hours of treatment with LLL12, RNA was collected from cells using a RNeasy Kit (Qiagen, #74104).
Total RNA was extracted from cells using a RNeasy kit (QIAGEN) and reverse transcribed using a First-Strand cDNA Synthesis Kit (GE Healthcare Bio-Science Corp).
Total RNA was purified from the samples using a RNeasy Kit (QIAGEN, Hilden, Germany), and single-strand cDNA was synthesized from 1 µg of total RNA using a First-Strand cDNA Synthesis Kit (GE Healthcare Life Science, Uppsala, Sweden).
Total RNA was extracted from the cell pellets using a RNEasy kit (Qiagen) according to the manufacturers protocol and concentration measured.
RNAs were extracted from the organs sampled at various time points (1, 6, 12, 48 hours and 1 week post infection) using a RNeasy kit (Qiagen).
Total RNA was harvested using a RNeasy kit (Qiagen) and transcripts detected in a one-step RT PCR reaction using Quantitect SYBR green reagent (Qiagen).
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Total RNA was isolated from C. guilliermondii using an RNeasy kit (Qiagen, Valencia, CA).
After precipitation, RNA was purified using an RNeasy kit (Qiagen) as recommended by the manufacturer.
RNA samples were extracted from H1, H9 and H14 cells using an RNeasy kit (Qiagen).
Total RNA was isolated from cell lysates using an RNeasy kit from Quiagen (Valencia, CA).
RNA was cleaned using an RNeasy kit following manufacturer's recommendations (Qiagen).
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