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The glycoalkaloids α-solanine and α-chaconine were determined using a reversed phase C18 HPLC column with a photodiode array detector.
Separation was performed using a reversed phase C18 (4.6 mm × 150 mm i.d., 5 μm) with mobile phase of acetonitrile methanol-1acetonitrile methanol-1%in water of 30:10:60 (v/v/v) at flow rate of 1 ml/min.
HPLC analyses for individual carotenoids were carried out on Shimadzu LC20 AT controller system with PDA detector SPD-M20A, using a reversed phase Hibar 250 4 Lichrosorb C18 column (250 × 4.6 mm), 5 μm.
Alkyne-modified bombesin analog (RM2-alkyne, BBN) (Leu-Sta-His-Gly-Val-Ala-Trp-Gln-D-Phe-4-amino-1-carboxymethyl-piperidine-4-pentynoic acid) was synthesized with a peptide synthesizer (Rainin/Protein Technologies) and purified by high performance liquid chromatography (HPLC) using a reversed phase XTerra® preparative column (C18, 10 μm, 19 mm × 250 mm).
Analytes were separated using a reversed phase SupelcoSil LC-18-DB 58993 15columnmm×3um column (Supelco) heated to 30°C.
The supernatant was analyzed using a reversed phase C-18 HPLC-assay.
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This research uses a reversed phase separation/coacervation method to fabricate flexible PLA and PLGA: excipient implants with dichloromethane/mineral oil as solvent/non-solvent.
This analysis used a reversed phase HPLC column (YMC 2.1×100 mm C18) thermostated at 40°C.
We used a reversed phase high performance liquid chromatography (RP-HPLC) [ 16] to analyze GABA content in the rice samples.
The chromatographic separation was achieved using a reversed-phase C8 column, pre-column derivatization with 9-fluorenylmethoxycarbonyl chloride (Fmoc-Cl) and ultraviolet detection (λ = 254 nm).
Cholesterol and α-tocopherol content of brain tissue was determined by reversed-phase HPLC using a C18 reversed phase column as described previously [54].
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