Sentence examples for using a reverse transcriptase from inspiring English sources

Gene expression was performed using a reverse transcriptase polymerase chain reaction (RT-PCR).

Swabs were tested using a reverse transcriptase polymerase chain reaction (RT-PCR) test.

Reverse transcription (RT) was carried out using a reverse transcriptase kit (Toyobo, Tokyo, Japan); real-time PCR was performed with an Eppendorf MasterCycler® ep RealPlex4 (Wesseling-Berzdorf, Germany).

Plasma HIV-1 viral load was measured using a reverse transcriptase (RT) polymerase chain reaction (PCR) technique (Amplicor, Roche Monitor-v1.5) from cryopreserved samples.

By simple calculations, using a reverse transcriptase error rate of 3.4×10−5 mutations per site and generation [3], [4] and viral production rate of 1010 [7] it can be estimated that all nucleotides in the HIV-1 genome on average mutate about 105 times per day in an HIV-1 infected individual.

First-strand complementary DNA was reverse-transcribed using a Reverse Transcriptase MIX Kit (Toyobo, Osaka, Japan).

Quantification of RNA was done using a Reverse Transcriptase-Polymerase Chain Reaction assay, with a lower detection limit of less than 1000 copies/mL.

The 5' coding region was subsequently isolated using a reverse transcriptase-PCR approach and was fused to one of the partial cDNAs at a common restriction site to produce a cDNA with full coding potential.

Both types use a reverse transcriptase, or RNA-dependent DNA polymerase enzyme, to carry out the nucleic acid conversion.

Also, it is possible to use a reverse transcriptase enzyme with increased thermal stability, since the reverse transcriptase template switching is usually not detected anymore in this case [ 97, 99].

The cDNA encoding for α-1,3-arabinofuranosidase was reverse by reverse-transcriptase-PCR using a reverse-transcriptase (Cat, No, M531A, Promega) and oligo-dT primer.