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Level of encapsulated DTX was measured using a reverse phase HPLC method.
HPLC was performed using a reverse phase C18 column (JT Baker, Bakerbond®, 4.6 × 250 mm) eluted with 0.1% TFA and methanol [18].
Compound 5 (69.5 mg) was purified from F1.4 by using a reverse phase C18 column eluted with acetone water (2 1 v/v).
Compound 1 (70.0 mg) and 4 (18.2 mg) were isolated from F1.7 by using a reverse phase C18 column eluted with acetone water (3:2 v/v).
The formation of 8-oxo-dG in the lung DNA was assessed using a reverse phase HPLC system coupled with UV and electrochemical (EC) detection.
The HPLC analyses were carried out on the Agilent 1200 system with a UV vis detector, using a reverse phase Eclipse XDB-C18 column (150 × 4.6 mm), 5 μm.
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Initially, biotinylated liposome nanocarrier encapsulated with glucose was synthesized using a reverse-phase evaporation method.
The samples were quantified by HPLC (LC 1100, Agilent Technologies, Santa Clara, CA, USA) using a reverse-phase C-18 column at 25 °C.
The reaction product 3 was analysed using a reverse-phase HPLC column: Purospher® STAR RP-18e (250 mm × 4,6 mm i.d., 5 μm) column (Merck).
Measurements and Main Results: Glutamate concentrations were determined using a reverse-phase high-pressure liquid chromatography method coupled to precolumn derivatization of the analyte with o-phthalaldehyde.
In the reaction with -alliin, the remaining concentrations of -alliin and -alliin were measured by HPLC using a reverse-phase C18 column (YMC-Pack ODS-AM, YMC Co., Kyoto, Japan) at 220 nm.
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