Sentence examples for using a reverse database from inspiring English sources

Data was then subjected to a second interrogation using a reverse database to further eliminate non-mycobacterial specific spectra.

False discovery rates were also estimated using a reverse database as decoy.

To ensure specificity, we apply a standard decoy database strategy (Bradshaw et al., 2006) using a reverse database.

Using a reverse database it is possible to calculate a false discovery rate (FDR) from matches to the forwards and decoy (reverse) database above a given APS protein threshold.

Using a reverse decoy database approach, the dataset was then filtered to a 1% false discovery rate (FDR); approximately 2000 proteins were identified with high confidence.

PSMs were filtered using Percolator (implemented in Proteome Discoverer) to control false discovery rates (FDR) to <1% as determined using a reverse-sequence decoy database.

Total RNA was extracted using Trizol reagent and reverse transcribed using a Reverse Transcription System (Promega).

After the peptide mixture is separated using a reverse-phase liquid chromatography electrospray ionization mass spectrometer, the peptides are identified by sequence database analysis [ 16].

The pipeline uses a concatenated reverse database [36] to estimate the FDR and incorporates filtering steps that target pseudogenes, repeat elements and sequence conservation across genomes to find additional support for the assignments made by the database search algorithm.

A reverse database strategy [33] was employed by concatenating reversed protein sequences for each database entry in SEQUEST.

The combined database was concatenated with a reverse database composed of all protein sequences in reversed order.