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An MS initiates a call by using a reverse control channel to make a request.
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The same was true using a reverse Aβ42-1 an additionalnal control.
RNA samples were checked for genomic DNA (gDNA) contamination using a control cDNA synthesis reaction without reverse transcriptase.
Previous research has studied the effects of table inclination on IOP in awake volunteers; however, the effects in spine surgery patients have not been investigated for reverse Trendelenburg positioning using a prospective, randomized controlled study design.
The aim of our study was to develop rapid diagnostic procedures of RVC by using an internal positive control of reverse transcription PCR (RT-PCR).
In addition to a library from ChIPs against SRF, we used a negative control library of reversed cross-links and no immunoprecipitation (RX-noIP) as a reference.
One study using a longitudinal design and controlling for reverse causality showed that childhood overweight contributed to a delay in motor development, but the survey sample was limited to low-income African-American infants from 3 to 18 months of age [ 22].
Any doubts that Mr. Hussein himself was involved appeared to disappear when the older of his two powerful sons, Uday Saddam Hussein, a maverick figure who has often intervened to reverse government policies, used a newspaper he controls in Baghdad today to chastise the Information Ministry for overreacting in its handling of CNN.
Possible genomic DNA contamination was excluded by using negative control (reverse transcriptase polymerase was excluded during RT-PCR) of RT-PCR samples (Figure S3).
Briefly, 100 ng of total RNA containing spiked in Poly-A RNA controls was used in a reverse transcription reaction (GeneChip 3′ IVT Express Kit Affymetrixx) to generate first-strand cDNA.
A tube containing equivalent amount of total RNA was used as reverse transcriptase control and amplified in the same PCR reaction.
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