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Expression was inversely correlated with the number of HSCs; therefore, ectopic expression of Lxn using a retroviral vector decreased stem cell population size.
Here we report the use of a T cell display system, using a retroviral vector, for generating a high-affinity TCR from the mouse T cell clone 2C.
Methods: Spleen-derived CD4+ cells were transduced by using a retroviral vector to simultaneously express IL-10 and green fluorescent protein (GFP).
We were able to efficiently transduce the MDSCs and all the other evaluated populations of MDCs (efficiency of transduction = approximately 80%) by using a retroviral vector expressing human bone morphogenetic protein 4 (BMP4).
Similar results were observed by using a retroviral vector for expression of the CFTR cDNA.
For in vivo visualization of grafted stem cells, F3 cells were genetically engineered using a retroviral vector (kindly provided by Dr. Brian Rabinovich of MD Anderson Cancer Center).
Similar(37)
To understand better the mechanisms by which syndecan-1 restrains repair, we used a retroviral vector to create BEAS-2b cells (immortalized human bronchial airway epithelial cell line) that stably expressed shRNA that complements either human syndecan-1 mRNA (B2bshRNA.Sdc1) or a nonsense (luciferase) mRNA (B2bshRNA.luc).
83 A second study used a retroviral vector in neonatal MPS VI cats.
A more recent study used a retroviral vector to restore E47 expression in splenic B cells from old mice.
Our strategy to identify AECA target molecules is to use a retroviral vector system and flow cytometry.
To identify dividing cells and their progenies, we used a retroviral vector derived from the Moloney murine leukemia virus (MoMuLV), containing a red fluorescent protein (RFP -expression cassette undeRFP -expressionf the cassettelovirunderrly enhancer and chicken betheacontrolofothe (cytomegalovirussly dearlybenhancerande et al. 2006; Zhao et al. 2006).
More suggestions(15)
using a random vector
using a retroviral over-expression
using a retroviral transduction
using a modified vector
using a lossless vector
using a human vector
using a retroviral insertion
using a retroviral packaging
using a pcDNA3 vector
using a structural vector
using a pGEX vector
using a binary vector
using a retroviral retroviral
using a retroviral expression
using a retroviral system
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