Sentence examples for using a retroviral system from inspiring English sources

Similarly, using a retroviral system that allowed expression of SYT-SSX2 in U2OS cells (human osteosarcoma) at levels similar to those detected in primary synovial sarcoma cells (Syn1 cells- Figure 1A; in synovial sarcoma, SYT-SSX2 expression is driven by the robustly active promoter of SYT), we were able to detect substantial colocalization of SYT-SSX2 with the polycomb protein Bmi1 (Figure 1B).

The wild-type cavin-3 or EGR1 was stably expressed in SV589 fibroblasts, H1299 cells and Cavin-3 KO MEFs using a retroviral system as previously described (Zhao and Michaely, 2008).

In 2007, human iPSCs were first generated by the same group by transducing adult human dermal fibroblasts with viral vectors carrying the key pluripotency genes, Oct3/4, Sox2, Klf4, and c-Myc (Yamanaka factors), using a retroviral system [ 26].

Cell immortalisation can occur spontaneously, however we used a retroviral system to immortalise cells via the transfer and stable production of the Simian virus 40 Large T antigen and the human telomerase reverse transcriptase protein.

Stable knockdown cells were created using a retroviral transduction system.

To this end, we transfected BaF/3 cells with a human T cell cDNA library using a retroviral transduction system.

In this study, we evaluated the HA and NA matching patterns of two pandemic H1N1 viruses (1918 and the current 2009 strain) and a highly pathogenic avian influenza A virus (H5N1) using a retroviral pp system.

Both the SV40T and hTERT genes were introduced into bat cells using a retroviral vector system, which results in the stable integration of the introduced genes into the cellular chromosomal DNA [27].

To directly address the involvement of NF-κB in the delay of cell death, nfkb−/− cells were reconstituted, using a retroviral transgenic system, with either rela transgene (Tg), which is the major transactivating subunit of NF-κB, or a DNA defective binding mutant of rela (R35AY36A Tg).

PDZ-RhoGEF was introduced to MEFs using a retroviral packaging system.

In summary, using a retroviral mobilization system, we have been able to efficiently recruit various mRNAs into retroviral particles.