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By searching for a deregulated LFA-1 phenotype using a retroviral insertion mutagenesis approach, Cherry et al. found that a decrease of RhoH expression activated LFA-1, thus rendering the leukocytes adhesive and therefore active.
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We extend our earlier work by using a retroviral gene-trap vector to generate insertions in >98% of the genes expressed in a human cancer cell line that is haploid for all but one of its chromosomes.
We expressed this TCR using a retroviral expression vector in human Tregs from peripheral blood.
An early human NK3.3 reporter cell line can be genetically modified to express CD4zeta using a retroviral transduction approach.
Similar results were observed by using a retroviral vector for expression of the CFTR cDNA.
For in vivo visualization of grafted stem cells, F3 cells were genetically engineered using a retroviral vector (kindly provided by Dr. Brian Rabinovich of MD Anderson Cancer Center).
Stable knockdown cells were created using a retroviral transduction system.
F3 hNSC was genetically modified with a mouse Akt1 gene using a retroviral vector.
Four independent hMSC isolates were infected with corresponding retroviruses using a Retroviral Gene Transfer and Expression system.
By using a retroviral vector for delivery, we were able to target newborn neurons.
In summary, using a retroviral mobilization system, we have been able to efficiently recruit various mRNAs into retroviral particles.
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