Sentence examples for using a retroviral construct from inspiring English sources

AES was expressed in SK-N-MC Ewing's sarcoma cells using a retroviral construct (Figure 4A).

The stable myristoylated AKT HA-tagged (MAH) cell line was generated using a pLNCX retroviral construct containing myristoylated HA-tagged AKT1 purchased from Addgene (Cambridge, MA).

A retroviral construct for Δ133p53 overexpression was generated by inserting the Δ133p53 cDNA into pQCXIN vector (BD Biosciences).18 The Δ133p53 cDNA was also cloned into pLenti6.3/TO/V5-DEST vector (Thermo Fisher Scientific) by using Spe I and Mlu I sites.

A retroviral construct encoding a full length murine Rb [41] was kindly provided by Kostis Alevizopoulos.

We created a retroviral construct that expresses an epitope-tagged version of ISG15 3XFlag-6His-ISG15 3XFlag-6His-ISG15 3XFlag-6His-ISG15

Hence, we introduced the C/EBPα gene into the cells by a retroviral construct carrying the rat C/EBPα cDNA.

We therefore generated MUTZ3 progenitor cells stably expressing hTERT via transduction with a retroviral construct encoding hTERT (designated hTERT-MUTZ3).

To induce ras expression, cells were infected with a retroviral construct containing ras (gift of Stuart A Aaronson, Mount Sinai School of Medicine, New York, NY, USA).

MCF-7 stable cell line with FOXO3a downregulated (MCF7-d8_pa) was generated by retroviral transduction with a retroviral construct containing small-hairpin RNA interference targeting FOXO3a (V2HS_169297; Open Biosystems, Huntsville, AL, USA).

We expressed this TCR using a retroviral expression vector in human Tregs from peripheral blood.

Similar results were observed by using a retroviral vector for expression of the CFTR cDNA.