The stimulatory effect of 17βE2 on MS-KIF18A mRNA expression led us to analyze the transcription regulation using a reporter gene assay.
One such assay, using a reporter gene that marks a telomeric tract, has been described above (Dunham et al., 2000).
The utility of cDNA microarray information for the design of cell-based sensor using a reporter gene approach is discussed.
In vitro evaluation using a reporter gene assay in Escherichia coli led to the discovery of the first competitive PqsR antagonists, which are highly potent (Kd,app of compound 20: 7 nM).
Using a reporter gene assay with firefly luciferase as readout, significant reduction of TGFβ3 bioactivity was detected to occur both in serum containing medium (SCM) and serum free medium (SFM).
Effects of costunolide on cellular activation induced by a tumor-promoting phorbol ester 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetate 12-O-tetradecanoylphorbol-13-acetateS) gene in a human monocyTPAcell line THP-1.
We measured the stability of a 16.5-repeat polyGT tract by using a reporter gene (URA3-GT) in which the microsatellite was inserted in-frame into the yeast URA3 gene.
These reports have generally used direct ex vivo labeling of stem cells with FDG [15, 16] or indirect labeling using a reporter gene such as herpes simplex virus type I thymidine kinase (HSV1-tk) [17, 18].
This system had a 88-fold regulation of transcription of rps13 using a reporter gene in T. gondii [20].
Translational regulation was monitored using a reporter gene, and the effects of various combinations of native or mutated UTRs on RNA stability and translational regulation were characterized.
Differential translational regulation in exponentially growing versus stationary phase cells was studied by comparing translational efficiencies using a reporter gene system.
