We used a replacement strategy to disrupt the endogenous Puf1 (Figure 2A) or Puf2 (Figure 2B) gene copy by double crossover homologous recombination [25].
Using a "gene" replacement strategy, we show that Fab-7 boundaries from two closely related species D.erecta, D.yakuba, and a more distant species D. pseudoobscura are able to substitute for the melanogaster boundary.
As an alternative for the absence of PL proteins, it may be hypothesized that Sporothrix may digest pectin by using polygalactouronase (CAZy GH28) as a replacement strategy.
The C2H2 zinc finger Pita protein binds to several BX-C boundaries including Fab-7 and Mcp To study Pita functions, we have used a boundary replacement strategy by substituting modified DNAs for the Fab-7 boundary, which is located between the iab-6 and iab-7 regulatory domains.
We have used a gene replacement strategy to generate mice that express the human apoE3 isoform in place of the mouse protein.
To estimate the fitness effect of the synonymous mutations themselves, we used an allelic replacement strategy to introduce each synonymous mutation into the ancestral genetic background that lacked the Δ(PFLU4435 4466) mutation (see Methods).
However, a replacement strategy has to be developed.
Here, we use a gene-replacement strategy at the calyx of Held, a large CNS model synapse that expresses robust PTP, to study the molecular mechanisms of PTP.
Following a tournament, the neural controllers were ranked competitively according to how many games they won and the population was propagated using a mutation and replacement strategy.
The leu3+ gene, predicted to encode α-isopropylmalate synthase (Figure 1), was targeted for deletion using a homologous recombination replacement strategy.
To test whether a deletion of cbeI would alleviate restriction of DNA from E. coli in C. bescii and allow transformation of unmethylated DNA, we constructed a chromosomal deletion of cbeI (Cbes2438) in JWCB005 [ 13], using a targeted marker replacement strategy previously described [ 14].
