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Using a relaxed molecular clock, the divergence between the eastern and western species was dated at 25.60 M years, which is considerably older than previously suggested.
This drawback is, to some extent, avoided by using a relaxed molecular clock, which allows the rate to vary among lineages.
In fact, Bayesian divergence dating analyses using a relaxed molecular clock indicate that it is one of the earliest diverging lineages of the entire North American clade (Figs. 1b,c).
To infer how the demographic history of each subtype changed through time we inferred Bayesian skyline plots using a relaxed molecular clock and the substitution rates estimated previously under the best-fitted model of nucleotide substitution (that is, GTR+Γ+I+SRD112 for HCV-1a, and 1b and HKY+SRD112 for HCV-3a).
Here, we apply Bayesian phylogenetic analyses using a relaxed molecular clock to demonstrate that the island of Bermuda, although no older than two million years, is home to the only extant representative of one of the earliest mainland North American Plestiodon lineages, which diverged from its closest living relatives 11.5 to 19.8 million years ago.
Divergence time estimates among seahorse lineages were obtained using a relaxed molecular clock method [ 58].
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Consequently, as suggested by Drummond et al. [ 50], we used a relaxed molecular clock.
Therefore, we used a relaxed molecular clock model for the molecular dating analysis described in the following section.
In addition, we used a relaxed molecular clock timeframe to compare tenrec evolutionary patterns with defined adaptive radiation characteristics.
The analysis was carried out without outgroups since BEAST does not rely on outgroups to root the tree; instead it uses a relaxed molecular clock.
To establish a quantitative framework for interspecies divergence in the Saccharomyces sensu stricto genus, we used a relaxed molecular clock approach to estimate the relative divergence times among lineages (Drummond et al. 2006).
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