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The missing part of this exon in rps3-2 was not found elsewhere in either cotton mitochondrial genome, even when using a relaxed BLAST of 1e−10 to 1e−6.
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BLAST searches were performed using NCBI BLAST 2.2.19 [ 80] and the GenBank, EMBL, DDBJ, and PDB databases as of Oct.28, 2009, using a relaxed E-value cutoff of E = 0.02.
Substitution rates were estimated using a relaxed uncorrelated lognormal molecular clock [34].
Here, the ellipses were calculated using a relaxed criterion, outlining an area of just 50% probability.
Divergence time estimates among seahorse lineages were obtained using a relaxed molecular clock method [ 58].
Continue moving up scales a half-step at a time, using a relaxed jaw and tongue.
Relaxed on-demand expansionThe second improvement is to use a relaxed on-demand expansion that reduces the need of exhaustive expansion and sorting operations.
We used a relaxed clock model, drawing substitution rates from a lognormal distribution.
Consequently, as suggested by Drummond et al. [ 50], we used a relaxed molecular clock.
Use a relaxed, friendly tone when making your call.
The resulting sequences were compared to GenBank using a BLAST program: the nucleotide blast.
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