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The absorbance of the resulting solution was quantified spectrophotometrically at 570 nm, using a reference wavelength of 630 nm.
We dissolved the formazan dye in DMSO and measured optical density at 540 nm using a reference wavelength of 620 nm.
A 200 μl portion was transferred to a 96-well plate and attenuance was measured at 540 nm using a reference wavelength of 690 nm (Spectramax Plus; Molecular Devices).
The wells were then emptied and the blue formazan salts were dissolved in acidic isopropanol (400 μl/well) and absorbance was measured using a plate reader (Biotek) at 540 nm using a reference wavelength of 690 nm.
The plate was washed thoroughly, incubated with 2,2′-azino-di- 3-ethylbenzthiazoline sulfonate) diammonium salt and absorbance was measured at 405 nm, using a reference wavelength of 490 nm (DTX 880 Multimode Reader, Beckman Coulter Inc., Brea, CA, USA).
To improve the current glucose detection limit of the sensor simple measurements using a reference wavelength (i.e., with no glucose absorption) or an out-of-phase excitation at a reference wavelength could be envisaged.
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The absorbance at 655 nm was used as a reference wavelength.
The reading at 690 nm was used as a reference wavelength to calculate a corrected absorbance (A550 – A550).
Results were read at a Fam wavelength (emission 516) by using Rox as a reference wavelength (emission 610).
The absorbance was read at 570 nm using 600 nm as a reference wavelength.
Absorbance was measured at 570 nm in a microplate reader (Tecan, Austria) using 630 nm as a reference wavelength.
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