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The results indicate that either calculation of approximated IDVG exhibits a close linear correlation with original IDVG measured using a reference glucose analyzer, although they are not interchangeable.
Original IDVG was calculated using a one-compartment model from serial incremental arterial plasma glucose concentrations above preinjection using a reference glucose analyzer.
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To improve the current glucose detection limit of the sensor simple measurements using a reference wavelength (i.e., with no glucose absorption) or an out-of-phase excitation at a reference wavelength could be envisaged.
Fructosamine-glucose discordance (measured-predicted glucose) was calculated using a reference equation (8).
where AAC was the overall area above the plasma glucose levels vs. time curve calculated by the trapezoidal method using a reference line obtained from the base control.
We initialized the closed-loop system by using approximate body weight and a reference glucose level.
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The BGMs used were successfully tested for accuracy at very high altitude (5,000 m) as previously reported (13) and tested regularly at different altitudes using standard reference glucose solutions (NOVA Biomedical, Waltham, MA).
In addition, we calculated approximated IDVG from the increase in plasma values above baseline at 3 min after glucose injection determined using the reference glucose analyzer Each approximated IDVG was calculated according to the following formula (proposed by us [ 9]; Table 2): approximated IDVG (l) = 24.4 × exp -0.03 × Δgl) + 2.7.
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