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Using a reference dataset we can define the generalisation hierarchy based on the coverage of the hypotheses.
We have compared the respective performances of each method using a reference dataset of 30 archaeal and 30 bacterial genomes in addition to simulated and real metagenomes.
We phased the individuals using a reference dataset of individuals which did not contain any individuals that are related to the ones we are phasing.
Such unidentified sequences belonging to the widely distributed ectomycorrhizal fungal genus Inocybe (Basidiomycota) were retrieved from GenBank and divided into species that were identified in a phylogenetic context using a reference dataset from an ongoing study of the genus.
We note that by using a reference dataset, we can predict haplotypes (or phase) at the common sites even for a single individual given the genotypes at the common sites for the individual.
Taken together, these data show that careful assessment of the parasite stage of development (age) at any experimental time point using a reference dataset is essential in ex vivo analyses of Plasmodium parasites.
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In our approach we propose to use a reference dataset so we can define a generalisation order based on the coverage of the hypotheses within this dataset.
To illustrate the AGEP approach, we used a reference dataset consisting of normalized Affymetrix gene expression microarray profiles from 1654 normal samples corresponding to 44 distinct healthy tissues types from the GeneSapiens database [ 7].
Using a reference transcription dataset that comprises RNA-seq data for liver and 15 additional human tissue types (E-MTAB-513) we confirmed the above observation and found that the gene expression association with liver CRMs, and to a lesser extent Singletons, was tissue-specific.
The RDP bacterial sequences were used as a reference dataset to determine the coverage rates of the known primers.
The National Heart Lung and Blood Institute Exome Sequencing Project Exome Variant Server (http://evs.gs.washington.edu/EVS/) (Feb 2012) was used as a reference dataset for rare variant allele frequency in a European American population (table 2).
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