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Fluorescence spectra were recorded on a Cary Eclipse fluorimeter (Agilent Technologies, Stockport, UK) using a reduced pathlength quartz cuvette (4 mm excitation/10 mm emission).
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UV/Vis spectra were recorded on a Perkin Elmer Lambda 35 UV/Vis double-beam Spectrophotometer at 25 °C in the wavelength range of 200 800 nm using a quartz cuvette (pathlength of cuvette was 10 mm).
CD spectra were recorded at 20°C in a JASCO J-600 spectropolarimeter using a 0.02 cm pathlength quartz cells with a protein concentration of 1.3 mg ml−1, and the spectra analysed using the SELCON3 [45], [45] procedure to estimate secondary structure content.
CD spectra were recorded at 25°C on a JASCO J-815 CD spectropolarimeter (JASCO, Easton, MD, USA) using a 1 mm pathlength cuvette and a step of 0.1 nm, a scan rate of 50 nm.s−1 and a scan average of four.
Far-UV CD data were collected from 280 nm to 185 nm at a scan rate of 50 nm/min and at 1 nm intervals using a 0.1 cm pathlength cell, a bandwidth of 2.5 nm, with an averaging time of 8 s.
All measurements were conducted at room temperature using a 1 cm pathlength cuvette.
Sample sizes of 2 μL were loaded onto the sample mount and then analyzed using a pathlength of 0.7 mm.
The spectra were recorded at wavelength between 190 and 250 nm using a 0.1 cm pathlength cell.
All samples were measured in an AvaSpec-2048 fiber optic spectrophotometer using a 10 mm pathlength quartz cuvette at a protein concentration of 5 µM and 2 µM for Sso TFEα/β and C62/39, respectively.
All spectra were measured using a 1 mm pathlength quartz cuvette, 1 nm step size, and 2 nm slit width in buffer A for Ure21−93-AP Ure21−93-APB for Ure21−80-HRP, using protein concentrandons of 6 and 3 μ m, respectively.
All CD data collection was performed on an Aviv model 420 spectrometer fitted with a total fluorescence accessory module and thermoelectric cuvette holder using a 1 mm pathlength quartz cuvette.
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