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We repeated the experiment using a reduced collagen induction schedule, featuring a reduced booster injection (reduction to 80%, 0.04 ml emulsion instead of 0.05 ml).
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Adding toremifene, an anti-estrogen triphenylethylene derivate, to desmoid fibroblasts reduced collagen accumulation by decreasing mRNA expression and increasing collagen degradation.
Together these results suggest that depletion of host (stromal) MMP13 – a key collagen degrading enzyme – increased total collagen I content, but reduced collagen I organization, in the periphery of E0771 mammary tumors grown in MMP13 KO versus WT mice.
However, HRP rats showed reduced collagen deposition.
A standard curve reflecting collagen amount was generated using a Sircol collagen standard (Biocolor).
Soluble collagen concentration in the cell culture medium was determined using a Sircol collagen assay 72 hours after paclitaxel exposure.
To use a novel computational approach to examine the molecular pathways involved in cartilage breakdown and to use computer simulation to test possible interventions for reducing collagen release.
Somebody else asks about decongestants; a young woman inquires about the risk of birth defects while using a collagen stimulator.
Previous experimental data (not used in the construction of the model) confirmed that p38 inhibition reduces collagen release in a bovine model of cartilage breakdown (34).
Pertinent to the regulation of collagen in the prostate, androgen deprivation induces marked morphological changes in rat prostatic smooth muscle cells [62], [63] reduces collagen synthesis, and induces a collagen fibrillar reorganization in the rat ventral prostate [64], [65], [66].
A previous study had reported that MTA could reduce collagen gene expression in primary hepatic stellate cells [56].
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