Sentence examples for using a reconstituted system from inspiring English sources

Using a reconstituted system, we demonstrate that complexin and synaptotagmin simultaneously bind to neuronal SNARE complexes and that both apo-synaptotagmin and complexin inhibit SNARE-mediated membrane fusion.

We tested a possible function of native α-Syn using a reconstituted system consisting of v-vesicles that mimic synaptic vesicles, and t-vesicles that mimic the presynaptic plasma membrane.

Experiments performed using a reconstituted system and a continuous flow reactor have shown that drag-in of rapidly consumable coagulation factor predecessors (such as factor VIII) can be very important and enhance coagulation [ 18].

By studying a native, actively translating ribosome, it was possible to obtain high-resolution information for the conformation of an A/P tRNA and polypeptide within the exit tunnel, two states that are particularly challenging to capture using a reconstituted system.

Using a reconstituted system, Iris Behrmann (Luxemburg City, Luxemburg) demonstrated that downstream of the common γ chain, which for example is part of the IL2-R, Jak 1 has a dominant role over Jak3, even though data on Jak3 deficiency had predicted the opposite [ 43].

Our analysis of minus strand transfer using a reconstituted system shows that the transfer is cation-dependent and that transfer efficiency decreases in the presence of lithium ions, which destabilize G-quadruplex structure, although the effect is less dramatic than observed for G-rich regions of gag and cPPT.

To explore the effect of cotransin on TMD integration, we used a reconstituted system comprising a mammalian translation extract supplemented with microsomal ER membranes (Sharma et al., 2010).

In order to evaluate the influence of G-quadruplex formation on strand transfer efficiency (the recombination reaction), we used a reconstituted system consisting of HIV-1 RT, HIV-1 nucleocapsid protein (NC), a primer (DNA oligonucleotide), and two RNA templates representing the two copies of the HIV-1 RNA genome.

Here, we present experiments using a reconstituted fusion system that suggest a simple model in which the complexin accessory helix forms an alternative four-helix bundle with the target-SNARE near the membrane, preventing the vesicle-SNARE from completing its zippering.

Having shown the ability of rIciA to inhibit helix opening in-vitro, experiments were designed to assess the ability of rIciA to actually inhibit DNA replication by using a reconstituted replication system.

Recent progress in the field of genetic code reprogramming using a reconstituted cell-free translation system has made it possible to incorporate a wide array of non-proteinogenic amino acids, including N-methyl-amino acids and D-amino acids.